trizol (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher trizol
Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trizol/trizol+reagent/10__22175_slash_mmb__17646-54-16-18
Average 90 stars, based on 1 article reviews

trizol - by Bioz Stars, 2026-09

90/100 stars

Images

RNA Extraction:

Article Title: Title Pending 17646
Article Snippet: .. RNA extraction and gene expression analysis Total RNA was extracted from 0.1 g of tissue using Trizol® (InvitrogenTM, Thermo Fisher Scientific®, Oregon, USA) following the manufacture’s recommendations. .. The total RNA was quantified with a NanoDrop spectrophotometer (Thermo Fisher Scientific®, Waltham, MA, USA), ensuring an optimal 260/280 ratio between 1.8 and 2.0, and the integrity was assessed in a 1% agarose gel.

Gene Expression:

Isolation:

Article Title: Fat mass and obesity–mediated N 6 -methyladenosine modification modulates neuroinflammatory responses after traumatic brain injury
Article Snippet: .. Total RNA was isolated with TRIzol (Invitrogen) following the manufacturer’s instructions and RNA quality was measured by using a NanoDrop3000 (ThermoFisher Scientific). .. The m 6 A RNA methylation quantification kit (Abcam) was used to measure the m 6 A content of the RNA.

Article Title: Loss of CCDC188 causes male infertility with defects in the sperm head–neck connection in mice
Article Snippet: .. RNA was isolated and purified from multiple adult tissues and testes from 0 to 35 days after birth from C57BL/6N mice with TRIzol (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was performed using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) with an oligo (dT) primer.

Purification:

Article Title: Fat mass and obesity–mediated N 6 -methyladenosine modification modulates neuroinflammatory responses after traumatic brain injury
Article Snippet: Exosomes were identified by western blot analysis of exosomal markers TSG101 (1:1000, Abcam, Cambridge, UK) and CD63 (1:500, Abcam). .. Total RNA from brain tissues or BV2 cells was purified using TRIzol (Invitrogen) and reverse transcribed using ABI reverse transcriptase (ABI, ThermoFisher Scientific), oligo (dT) primers, and specific RT primers. ..

Reverse Transcription:

Article Title: Fat mass and obesity–mediated N 6 -methyladenosine modification modulates neuroinflammatory responses after traumatic brain injury
Article Snippet: Exosomes were identified by western blot analysis of exosomal markers TSG101 (1:1000, Abcam, Cambridge, UK) and CD63 (1:500, Abcam). .. Total RNA from brain tissues or BV2 cells was purified using TRIzol (Invitrogen) and reverse transcribed using ABI reverse transcriptase (ABI, ThermoFisher Scientific), oligo (dT) primers, and specific RT primers. ..

cDNA Synthesis:

Article Title: The complex role of protocadherin-19 in brain function: a focus on the oxytocin system
Article Snippet: .. mRNA was extracted from the hippocampus and cerebral cortex of Pcdh19 cKO mice and controls of both sexes at PND 27 using Trizol (Invitrogen). mRNA was retrotranscribed into cDNA using Maxima H Minus cDNA Synthesis Master Mix, with dsDNase (ThermoFisher). .. Oxtr, Avpr1a, and the housekeeping gene Gapdh were amplified by using TaqMan Universal Master Mix II with UNG (Applied Biosystems) in the Quantum Studio7 thermocycler (Applied Biosystems).

Review